首页|Effects of sonication on the in vitro digestibility and structural properties of buckwheat protein isolates

Effects of sonication on the in vitro digestibility and structural properties of buckwheat protein isolates

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  • NSTL
  • Elsevier
The present work investigated the effects of sonication at different amplitudes and durations on the in vitro digestibility of buckwheat protein isolates (BPIs). The conformation, particle size and microstructures of the BPIs were also studied to explicate the possible mechanisms of the sonication-induced changes. The results showed that sonication conditions of 20 kHz, pulsed on-time 10 s, off-time 5 s, amplitude of 60% and duration of 10 min (SA6T10) improved the digestibility of BPIs from 41.4% (control) to 58.2%. The tertiary structure analysis showed that sonication exposed the hydrophobic core buried inside the protein molecules and broke the intramolecular crosslinks, based on the increase in the surface hydrophobicity and intrinsic fluorescence and the decrease in the disulphide content. The secondary structure analysis showed that SA6T10 decreased the content of beta-turn and beta-sheet by 40.9% and 22.4%, respectively, and increased the content of anti-parallel beta-sheet, random coil, and alpha-helix by 40.9%, 30.6%, and 25.5%, respectively. The particle size of the control BPIs (427.7 +/- 76.7 nm) increased to 2130.8 +/- 356.2 nm in the SA6T10 sonicated sample with a corresponding decrease in the polydispersity index from 0.97 +/- 0.04 to 0.51 +/- 0.13. Moreover, scanning electron microscopy indicated that sonication broke the macroparticles into smaller fragments and changed the surface state of the proteins. Taken together, sonication has proven to be a promising approach for improving the digestibility of buckwheat proteins, which can be explored as a source of plant-based alternative protein for food applications.

UltrasoundBuckwheatAlternative proteinDigestibilityStructural property

Jin, Jian、Okagu, Ogadimma D.、Yagoub, Abu ElGasim Ahmed、Udenigwe, Chibuike C.

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Southwest Univ Sci & Technol, Sch Life Sci & Engn, 59 Qinglong Rd, Mianyang 621010, Sichuan, Peoples R China

Univ Ottawa, Dept Chem & Biomol Sci, Fac Sci, Ottawa, ON K1N 6N5, Canada

King Saud Univ, Dept Food Sci & Nutr, Coll Food & Agr Sci, POB 2460, Riyadh 11451, Saudi Arabia

Univ Ottawa, Sch Nutr Sci, Fac Hlth Sci, Ottawa, ON K1H 8M5, Canada

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2021

10.1016/j.ultsonch.2020.105348

Ultrasonics sonochemistry

Ultrasonics sonochemistry

CCREI
ISSN：1350-4177
年,卷(期)：2021.70
  • 36
  • 45