Simple and reliable methods for the determination of sixteen marker components for quality control of Daochi pill by HPLC coupled with diode array detection
Simple and reliable methods for the determination of sixteen marker components for quality control of Daochi pill by HPLC coupled with diode array detection
1. College of Pharmacy, Dalian Medical University, 9 Western Lvshun South Road, Dalian 116,044, China
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Abstract
INTRODUCTION: Traditional Chinese medicine plays a very important role in the healthcare system of China and thus the quality control of medicinal herb products is of paramount concern. OBJECTIVE: To establish a simple and effective high-performance liquid chromatography (HPLC) method to evaluate the quality of Daochi pill. METHODOLOGY: Two HPLC methods were developed for the determination of 16 marker components in Daochi pills. In method A, the analytes were separated on a Lichrosorb C column using a gradient elution of methanol and 1% aqueous phosphoric acid (pH 2.9, adjusted by triethylamine). In method B, the separation was achieved on an Agilent Eclipse Plus C column using a gradient elution of methanol and 3% aqueous phosphoric acid (pH 2.0, adjusted by triethylamine) in a gradient elution mode. RESULTS: Methods were linear over the range 0.27-500 og/mL (rpo greater-than-or-equal 0.9995). Accuracy, precision and repeatability were all within the required limits. The mean recoveries measured at three concentrations were higher than 95% with RSD [less-than or equal to] 4% for the targets. CONCLUSION: The established methods are simple and can be used as tools for quality evaluation and control of this natural product. Copyright pb 2009 John Wiley '' Sons, Ltd.
Key words
Daochi pill/herbal preparation/HPLC/quality control
NSTL
Wiley