Asymbiotic seed germination of Coelogyne cristata Lindl. was accomplished in agar-gelled MS medium. Complete seedlings were obtained in 8 wks of primary culture. Multiple shoots (9.25 shoots/ plant) were developed from the shoot tip explant obtained from in vitro grown seedlings in MS medium supplemented with BAP (1mgl1) and NAA {0.5 mgl-1)-. Medium supplemented with IBA (1mgl'1) proved optimal for root formation. Thick hairy aerial roots were observed in the media supplemented with IAA (Imgl1).
B Pant、S Swar、A Karanjeet
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Central Department of Botany, Tribhuvan University, Kathmandu, Nepal
NSTL
