首页|C-Phycoycanin-Doxorubicin Nanoparticles for Chemo-Photodynamic Cancer Therapy
C-Phycoycanin-Doxorubicin Nanoparticles for Chemo-Photodynamic Cancer Therapy
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NSTL
Polymer Soc Korea
C-Phycocyanin (CPC) obtained from Spirulina platensis is a natural blue dye and is attracting attention due to its photodynamic activity, anti-inflammatory effect and anti-cancer properties. The aim of this study is to prepare the CPC-doxorubicin (CPC-DOX) nanoparticles and evaluate chemo-photodynamic therapeutic effects of the nanoparticles in a mouse model bearing CT26 colorectal cancer xenograft CPC-DOX conjugate prepared by amide coupling method was characterized by Fourier transform infrared spectroscopy. CPC-DOX conjugate was suspended in PBS buffer and sonicated for formation of self-assembled nanoparticles. The size distribution and morphology of CPC-DOX nanoparticles were analyzed by dynamic light scattering and transmission electron microscopy. Reactive oxygen species (ROS) generation from CPC-DOX nanoparticles was evaluated using 1,2-diphenylisobenzofuran (DPBF). To confirm chemo-photodynamic therapeutic effects of CPC-DOX nanoparticles, cell viability and cancer growth were investigated on CT26 cells and a CT26 xenograft mouse model. The CPC-DOX conjugate was successfully synthesized by amide coupling reaction. The conjugate formed self-assembled nanoparticles in aqueous solution. In generation of ROS using DPBF, CPC-DOX nanoparticles exhibited significantly higher DPBF photobleaching effect than CPC. CPC-DOX nanoparticles significantly decreased cell viability in CT26 cells by generation of singlet oxygen after 635 nm near-infrared (NIR) laser irradiation in vitro. CT26-bearing mice injected with CPC-DOX nanoparticles through the tail vein and irradiated with a 635 nm NIR laser showed the most cancer inhibition among all groups. In summary, nanoparticles formed from CPC-DOX conjugate have potential as novel chemo-photodynamic therapeutic agents for treating cancer.
NSTL
Polymer Soc Korea
