首页|HIV-1 Tat and cocaine impact astrocytic energy reservoir influence on miRNA epigenetic regulation
HIV-1 Tat and cocaine impact astrocytic energy reservoir influence on miRNA epigenetic regulation
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
? 2021 Elsevier Inc.Astrocytes are the primary regulator of energy metabolism in the central nervous system (CNS), and impairment of astrocyte's energy resource may trigger neurodegeneration. HIV infections and cocaine use are known to alter epigenetic modification, including miRNAs, which can target gene expression post-transcriptionally. However, miRNA-mediated astrocyte energy metabolism has not been delineated in HIV infection and cocaine abuse. Using next-generation sequencing (NGS), we identified a total of 1900 miRNAs, 64 were upregulated and 68 miRNAs were downregulated in the astrocytes by HIV-1 Tat with cocaine exposure. Moreover, miR-4727-3p, miR-5189-5p, miR-5090, and miR-6810-5p expressions were significantly impacted, and their gene targets were identified as VAMP2, NFIB, PPM1H, MEIS1, and PSD93 through the bioinformatic approach. In addition, the astrocytes treated with the nootropic drug piracetam protects these miRNAs. These findings provide evidence that the miRNAs in the astrocytes may be a potential biomarker and therapeutic target for HIV and cocaine abuse-induced neurodegeneration.
Astrocyte and cocaineHIV-1 TatmiRNAPiracetam
Doke M.、Kashanchi F.、Khan M.A.、Samikkannu T.
展开 >
Department of Pharmaceutical Sciences Irma Lerma Rangel College of Pharmacy Texas A&M University
National Center for Biodefense and Infectious Disease Laboratory of Molecular Virology George Mason
NSTL
Elsevier
