The Journal of surgical research.2022，Vol.2749.DOI:10.1016/j.jss.2022.01.004

Outcomes and Perioperative Nutritional Management in a Porcine Model of Short Bowel Syndrome

Tsikis, Savas Theodore Fligor, Scott C. Secor, Jordan D. Yu, Lumeng J. Pan, Amy Loring, Greta First, Eric Nedder, Arthur P. Grammer, Robin M. Rudie, Coral Gura, Kathleen M. Puder, Mark
The Journal of surgical research.2022，Vol.2749.DOI:10.1016/j.jss.2022.01.004
✕
  • NSTL
  • Elsevier

Outcomes and Perioperative Nutritional Management in a Porcine Model of Short Bowel Syndrome

Tsikis, Savas Theodore 1Fligor, Scott C. 1Secor, Jordan D. 1Yu, Lumeng J. 1Pan, Amy 1Loring, Greta 2First, Eric 2Nedder, Arthur P. 3Grammer, Robin M. 3Rudie, Coral 4Gura, Kathleen M. 5Puder, Mark1
扫码查看

作者信息

  • 1. Boston Childrens Hosp,Harvard Med Sch
  • 2. Alcresta Therapeut
  • 3. Anim Care Resources Childrens Hosp,Boston Childrens Hosp
  • 4. Dept Gastroenterol,Boston Childrens Hosp
  • 5. Dept Pharm,Boston Childrens Hosp
  • 折叠

Abstract

Introduction: Short bowel syndrome (SBS) results from significant intestinal loss and is characterized by insufficient absorption of nutrients and fluids. Preclinical large animal SBS models typically require parenteral nutrition (PN) support and may not be appropriate for studying interventions to improve intestinal absorption or adaptation. Here, we describe the development of a porcine SBS model that does not require PN support. Methods: Eight male Yorkshire piglets underwent either a 75% or 90% jejunoileal resection (n = 5) or no resection (n = 3). Continuous enteral nutrition (EN) was provided via a gastrostomy tube. The final SBS model consisted of a 75% resection and nutrition provided via combination EN (60%) and per oral pig chow (40%). Body weight and concentration of fat-soluble vitamins were assessed on postoperative days (POD) 7, 14, and 21. For assessing fat malabsorption, the coefficient of fat absorption (CFA) was calculated following a 72-h stool collection. Results: Resected animals had decreased weight gain compared to unresected controls (POD21 + 8.3% versus +28.8%, P = 0.048). Vitamin D concentration was significantly lower in resected animals compared to controls on POD 7, POD 14, and POD 21. Serum vitamin E concentration was also lower on POD 21. Resected animals developed fat malabsorption with lower CFA (76.5% versus 95.3%, P = 0.014). Conclusions: We describe the development of a porcine SBS model that does not require PN support. Piglets in this model gain less weight, demonstrate fat malabsorption, and develop fat-soluble vitamin deficiencies. This model will benefit investigations of intestinal absorption or adaptation while potentially decreasing costs and confounding complications related to PN administration. 2022 Elsevier Inc. All rights reserved.

Key words

Short bowel syndrome/Porcine model/Nutritional optimization/Fat-soluble vitamins/Steatorrhea/Coefficient of fat absorption/INTESTINAL ADAPTATION/PIGLET MODELS

引用本文复制引用

出版年

2022
The Journal of surgical research.

The Journal of surgical research.

ISSN：0022-4804
被引量3
参考文献量18
段落导航相关论文