首页|Identification of an ERN1 target site within EGFP mRNA
Identification of an ERN1 target site within EGFP mRNA
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NSTL
EGFP (enhanced green fluorescent protein) is one of the most common tools used in life sciences, including research focusing on proteostasis. Here we report that ERN1 (endoplasmic reticulum to nucleus signaling 1), which is upregulated by UPR (unfolded protein response), targets an RNA hairpin loop motif in EGFP mRNA. A silent mutation introduced into EGFP mRNA abolished the ERN1-dependent mRNA decay. Therefore, experiments that employ EGFP as a reporter gene in studies that involve upregulation of the UPR pathway should be interpreted carefully, and a mutant devoid of the ERN1 target motif may be more suitable for such studies.
Marius W. Baeken、Yohei Yokobayashi
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Nucleic Acid Chemistry and Engineering Unit, Okinawa Institute of Science and Technology Graduate
NSTL
