首页|Identification and function analysis of BmPxtA in the immune response regulated by PGE(2) of silkworm, Bombyx mori
Identification and function analysis of BmPxtA in the immune response regulated by PGE(2) of silkworm, Bombyx mori
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NSTL
Elsevier
Prostaglandins (PGs) can mediate the immune response of insects to infection. Mammalian cyclooxygenase (COXs) is a key enzyme in the synthesis of PGs, and Pxt may be its homologous gene in some sequenced insect genomes. As a representative of Lepidoptera, the silkworm also contains PGs, but the biosynthetic source of PGs is still unclear. In this study, Sequence analysis showed that peroxinectin (BmPxtA) gene of silkworm was closely related to human COX gene, and its homologous protein had conserved domains corresponding to human COX. The expression of BmPxtA gene was the highest in the hemocytes and was induced by Nuclear Polyhedrosis Virus (NPV) challenge in the detected tissues. The quantitative polymerase chain reaction (qPCR) results showed that silencing BmPxtA mediated by RNA interference (RNAi) inhibited the expression of immune-related pathway genes, and specifically suppressed hemocyte-spreading and nodule formation in silkworm; Hemocyte-spreading and nodule formation were also inhibited by aspirin, a COX inhibitor. Treatment by PGE2 but not arachidonic acid (AA) rescued the immunosuppression; PGs concentrations was also inhibited by aspirin. PGE2, but not AA, treatment rescued the PGs concentrations. These results suggest that BmPxtA gene is associated with PG biosynthesis in silkworm and the immune response of silkworm was affected by regulating the concentrations of PGs.
NSTL
Elsevier
