首页|A new near-infrared fluorescence indicator derived from chloro-substituted dicyanoisophorone for detecting carboxylesterases(CEs)in living cells and in vivo
A new near-infrared fluorescence indicator derived from chloro-substituted dicyanoisophorone for detecting carboxylesterases(CEs)in living cells and in vivo
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Carboxylesterase(CEs)are a kind of liver-synthesized esterase and play a great role in various physiological activities such as drug metabolism and transport processes.Accurate detection of their activity through near-infrared(NIR)fluorescence probe with large Stokes shift is still needed to investigate their biological function and diagnose related disease in vivo.In present study,a new"off-on"fluorescent probe(ZM-1)was constructed with large Stokes shift(160 nm).ZM-1 could be used to detect CEs with a bright NIR emission in a concentration dependent and time-dependent manner.The detection line of ZM-1 is as low as 0.287 × 10~3 U/mL.Moreover,ZM-1 possessed high selectivity and low toxicity.ZM-1 exhibited good optical performance to track CEs activity in cells and monitor the change of CEs activity on 5-Fu-induced model,indicating its good cell membrane permeability and precise detection character.More importantly,ZM-1 was successfully used to in situ image CEs activity in vivo.These studies suggest that probe ZM-1 has potential applicable valuation for CEs detection in living bio-systems and CEs-related disease diagnose.
NSTL
