Cytotoxic alkaloids from the root of Zanthoxylum paracanthum (mildbr) Kokwaro

Leonidah Kerubo,Omosa ¹Vaderament-A,Nchiozem-Ngnitedem ¹Justus,Mukavi ²Brenda,Atieno Okoko ¹Helder,Ombui Nyaboke ¹Ibrahim,Hashim ¹Jackson,Obegi Matundura ¹Thomas,Efferth ³Michael,Spiteller⁴

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作者信息

1. Department of Chemistry, University of Nairobi
2. School of Pure and Applied Sciences, University of Embu
3. Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes
4. Institute of Environmental Research (INFU), Department of Chemistry and Chemical Biology, Chair of
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Abstract

Abstract Chemical investigation of the root of Zanthoxylum paracanthum afforded 1 new alkamide derivative, (2E,4E)-6-oxo-N-isobutyldeca-2,4-dienamide (1) together with 10 known congeners including one phenolic amide (2), four benzophenanthridines (3???6), three indolonaphthyridines (7???9) and two lignans (10 and 11). Their structures were elucidated by a combination of spectroscopic and spectrometric data. Using resazurin reduction assay, the crude extract (10?μg/mL) and isolates (10?μM) were screened for their cytotoxic activities against the drug-sensitive (CCRF-CEM) leukemia cell line and its multidrug-resistant counterpart (CEM/ADR5000). Compounds 3, 4 and 6 showed cytotoxicity against CCRF-CEM with IC50 values of 2.00?±?0.33, 2.31?±?0.20 and 0.11?±?0.04?μM, respectively. Only compound 6 exhibited strong cytotoxic activity against CEM/ADR5000 with an IC50 value of 2.34?±?0.34?μM in comparison with the standard drug doxorubicin which showed IC50 values of 0.01?±?0.14 (CCRF-CEM) and 26.78?±?3.30?μM (CEM/ADR5000).

Key words

Zanthoxylum paracanthum/Rutaceae/alkamide/cytotoxicity

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出版年

2022

Natural product research

SCI

ISSN：1478-6419

被引量5

参考文献量31

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