首页|Dual-color simultaneous structured illumination microscopy based on galvo-mirrors

Dual-color simultaneous structured illumination microscopy based on galvo-mirrors

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Structured illumination microscopy (SIM) is one of the most widely used imaging tools in live-cell imaging because of the fast imaging speed and low-intensity requirement. In order to observe the interactions between cell structures, it is necessary to achieve multi-color simultaneous rapid imaging. However, existing systems collect multi-color results in a time-sequential manner. In live-cell imaging, this method brings distortions of the result. In this paper, we provide a dual-color simultaneous SIM system based on galvanometers, which can control the structured pattern of the two-channel flexibly. The resolution capability and simultaneous imaging capability of this system are verified, and the imaging results of living cells are obtained.

Fluorescence microscopySuper-resolutionLive-cell multicolor imagingStructured illuminationSimultaneous imagingFLUORESCENCE MICROSCOPYRESOLUTION LIMITCELLS

Yuan, Yifan、Liu, Wenjie、Wang, Yueying、Yang, Lu、Xu, Fanghui、Hao, Xiang、Han, Yubing、Kuang, Cuifang、Liu, Xu

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Zhejiang Univ

2022

Optics Communications

Optics Communications

EISCI
ISSN:0030-4018
年,卷(期):2022.511
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