首页|Formation of trifluoroacetic artefacts in gas chromatograph injector during Cannabidiol analysis
Formation of trifluoroacetic artefacts in gas chromatograph injector during Cannabidiol analysis
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NSTL
Elsevier
The knowledge of compounds stability in the process of sample preparation for analysis and during analysis itself helps assess the accuracy and precision of estimating their concentration in tested samples. The present paper shows that a significant amount of CBD present in the blood/plasma sample analyzed by means of GC transforms in the hot GC injector not only to 9 alpha-hydroxyhexahydrocannabinol, 8-hydroxy-iso-hexahydrocannabinol, delta-9-tetrahydrocannabinol, Delta 8-tetrahydrocannabinol, and cannabinol but also to the trifluoroacetic esters of Delta 9-THC and Delta 8-THC, when trifuoroacetic acid is used as protein precipitation agent. The amount of those newly revealed CBD transformation products depends on the GC injector temperature and on the extrahent type when extracts of the supernatants centrifuged from human plasma samples are analyzed after their preliminary protein precipitation by trifuoroacetic acid. Although trifuoroacetic acid as a protein precipitating agent has many disadvantages, it is quite often used for this purpose due to its very high protein precipitation efficiency. The results presented in the study demonstrate why the use of trifuoroacetic acid for plasma samples deproteinization should be avoided when CBD is determined by GC. (c) 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
CBD transformationTrifluoroacetic esters of THCPlasma precipitationGC-MSLIQUID-LIQUID-EXTRACTIONSAMPLE PREPARATIONHUMAN PLASMACANNABINOIDSMETABOLITESDELTA(9)-TETRAHYDROCANNABINOLPRECIPITATION
Holowinski, Piotr、Typek, Rafal、Dawidowicz, Andrzej L.、Rombel, Michal、Dybowski, Michal P.
NSTL
Elsevier
