首页|Anti-allergic potential of Typhonium blumei : Inhibition of degranulation via suppression of PI3K/PLC gamma 2 phosphorylation and calcium influx

Anti-allergic potential of Typhonium blumei : Inhibition of degranulation via suppression of PI3K/PLC gamma 2 phosphorylation and calcium influx

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  • NSTL
  • Elsevier
Background: Typhonium blumei Nicolson & Sivadasan (Araceae) is a traditional Chinese medicinal herb possessing detumescent, detoxifying, and anti-inflammatory activities. It is used in Taiwan as a folk medicine to treat cancer and inflammatory diseases. Typhonium blumei is usually not distinguished from Typhonium roxburghii Schott and they are commonly used interchangeably. Purpose: To evaluate and compare the anti-allergic and anti-inflammatory properties of T. blumei and T. roxburghii, their composition profiles and molecular basis of the anti-allergic effect. Methods: The methanolic plant extracts were partitioned with different solvents to obtain the nonpolar fractions. The anti-allergic activity of the nonpolar fractions was assessed by A23187-and antigeninduced degranulation assays using RBL-2H3 mast cells. Several molecular targets were investigated: Fc epsilon RI receptor expression by flow cytometry, calcium influx by live cells imaging fluorescent microscopy, cytokines mRNA expression by RT-PCR, and protein expression by Western blotting. The antiinflammatory activity was evaluated using superoxide anion and elastase release assays in human neutrophils. TLC, NMR and GC-MS analyses were conducted to evaluate the chemical composition of the fractions.

Typhonium blumeiTyphonium roxburghiiAnti-allergicAnti-inflammatoryFatty acidsCalcium influx

Korinek, Michal、Chen, Kuan-Ming、Jiang, Yu-Han、El-Shazly, Mohamed、Stocker, Joel、Chou, Chon-Kit、Hwang, Tsong-Long、Wu, Yang-Chang、Chen, Bing-Hung、Chang, Fang-Rong

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Kaohsiung Med Univ, Coll Pharm, Grad Inst Nat Prod, Kaohsiung 807, Taiwan

Kaohsiung Med Univ, Coll Life Sci, Dept Biotechnol, Kaohsiung 807, Taiwan

Natl Yang Ming Univ, Educ Ctr Humanities & Social Sci, Taipei 112, Taiwan

Chang Gung Univ, Coll Med, Grad Inst Nat Prod, Taoyuan 333, Taiwan

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2016

10.1016/j.phymed.2016.10.011

Phytomedicine

Phytomedicine

SCI
ISSN：0944-7113
年,卷(期)：2016.23(14)
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