首页|Extraction and characterization of an exopolysaccharide from a marine bacterium
Extraction and characterization of an exopolysaccharide from a marine bacterium
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Springer Nature
The marine bacterial exopolysaccharides (EPS) have transfigured the biotech sector with their myriad applications and prospects. This work was carried out to characterize and analyze the functional and biochemical properties of an EPS (EPS-DR3A) produced by a marine bacterium, Pseudoalteromonas sp. YU16-DR3A. The bacterium was cultured in Zobell marine broth for the production of EPS. The extracted EPS designated as EPS-DR3A was composed of 69% carbohydrates and 7.6% proteins with a molecular weight of 20 kDa. FT-IR spectra showed the presence of different functional groups. The monosaccharide analysis performed using GC-MS showed the presence of fucose, erythrotetrose, ribose, and glucose as monomers. EPS-DR3A showed excellent emulsifying activity against the tested hydrocarbons and food oils with stable emulsions. Rheological analysis of EPS-DR3A revealed the pseudoplastic behavior. The EPS-DR3A displayed good thermal stability with a degradation temperature of 249 degrees C and a melting point at 322 degrees C. Further, it had the ability to scavenge DPPH and nitric oxide free radicals with good total antioxidant activity. The in vitro biocompatibility study of EPS-DR3A showed high degree of biocompatibility with human dermal fibroblast cells at the tested concentrations. Taken together, the findings such as thermostability, emulsifying activity, pseudoplasticity, antioxidant activity, and biocompatibility of EPS-DR3A make this biomolecule an important candidate for a wide range of biomedical applications.
NSTL
Springer Nature
