首页|The enhancement role of Matrigel on HBV infection in HepG2-NTCP cells
The enhancement role of Matrigel on HBV infection in HepG2-NTCP cells
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NSTL
Elsevier
? 2021 Elsevier B.V.The hepatoma cell lines stably expressing sodium taurocholate cotransporting polypeptide (NTCP), the receptor of hepatitis B virus (HBV) infection, serve as important infection models for studying viral biology and drug discovery. However, the efficiency of infection greatly varies. In this study, we studied the effects and potential mechanisms of Matrigel? hESC-qualified (M-hq), a biological basement membrane matrix commonly used in cell culture, on promotion HBV in vitro infection in HepG2-NTCP cells. For the first time, our findings demonstrate that M-hq could enhance the infection efficiency of cell culture-derived HBV with no impact on the cell viability, the HBV transcription and response to antiviral treatments. The infection enhancement is reproducible and is suggested to occur at HBV attachment step. Our study suggests that this novel system is applicable for studying HBV biology and new drugs.
AttachmentHepatitis B virusHepatoma cell linesIn vitro infectionMatrigelSodium taurocholate cotransporting polypeptide
Song J.、Ou G.、Lai X.、Tian X.、Wang L.、Zhang K.、Zhang X.、Zhao C.、Zhuang H.、Xiang K.、Li T.
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Department of Microbiology and Infectious Disease Center School of Basic Medical Sciences Peking University Health Science Center
NSTL
Elsevier
