Russian journal of bioorganic chemistry2022，Vol.48Issue(4) ：8.DOI:10.1134/S1068162022040173

Bluetongue Virus Detection Using Microspheres Conjugated with Monoclonal Antibodies against Group-Specific Protein Vp7 by Flow Virometry

Rudenko, N., V Karatovskaya, A. P. Zamyatina, A., V Malogolovkin, A. S. Oleinikov, V. A. Brovko, F. A. Kol'tsov, A. U. Lapteva, O. G. Kolbasov, D., V Shepelyakovskaya, A. O.
Russian journal of bioorganic chemistry2022，Vol.48Issue(4) ：8.DOI:10.1134/S1068162022040173
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Bluetongue Virus Detection Using Microspheres Conjugated with Monoclonal Antibodies against Group-Specific Protein Vp7 by Flow Virometry

Rudenko, N., V 1Karatovskaya, A. P. 1Zamyatina, A., V 1Malogolovkin, A. S. 2Oleinikov, V. A. 3Brovko, F. A. 1Kol'tsov, A. U. 4Lapteva, O. G. 4Kolbasov, D., V 4Shepelyakovskaya, A. O.1
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作者信息

  • 1. Shemyakin Ovchinnikov Inst Bioorgan Chem
  • 2. Sirius Univ Sci & Technol
  • 3. Russian Acad Sci
  • 4. Fed Res Ctr Virol & Microbiol
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Abstract

Bluetongue is a reemerging transmissive infectious disease that threatens all countries with intensive livestock production. In cattle, bluetongue may either lead to lethality or be asymptomatic with a long-lasting viremic phase, which makes development of high-sensitivity bluetongue virus detection methods an important task. Methods of virus particle detection based on immunofluorescence assay in bluetongue-infected Vero cells, sandwich enzyme immunoassay and flow virometry have been developed using high-affinity monoclonal antibodies against VP7 (viral protein 7). High analytical sensitivity of the method (10(-0.25) TCID50/mL (tissue cytopathogenic dose)) was demonstrated in a flow cytometry assay. The ability to rapidly and efficiently detect virus particles in the biological fluids obtained from animals by flow virometry was demonstrated in a model system in which BTV was measured in cattle blood serum. The detection sensitivity was 10(0.75) TCID50/mL.

Key words

bluetongue virus (BTV)/viral protein 7 (VP7)/enzyme immunoassay/immunofluorescence/monoclonal antibodies/flow virometry/ENDOPEPTIDASES/IDENTIFICATION/IMMUNOASSAY/PARTICLES/ALPA

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出版年

2022
Russian journal of bioorganic chemistry

Russian journal of bioorganic chemistry

SCI
ISSN：1068-1620
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