首页|CRYOPRESERVATION OF SWEET POTATO USING VITRIFICATION TECHNIQUE: EFFECTS OF THE VITRIFICATION PROCEDURE AND SUCROSE PRETREATMENTS
CRYOPRESERVATION OF SWEET POTATO USING VITRIFICATION TECHNIQUE: EFFECTS OF THE VITRIFICATION PROCEDURE AND SUCROSE PRETREATMENTS
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NSTL
An effective vitrification procedure was developed for sweet potato cryopreservation, which was applied to three accessions (Tainung Nos. 25, 64, and 66). Effects of 0.4 M sucrose pretreatment as well as durations of loading and dehydration with plant vitrification solution 2 (PVS2) on the survival of shoot tips were examined. In the optimal vitrification procedure, explant pretreatment by stepwise increases in sucrose concentrations up to 1.0 M was used to investigate recalcitrant accessions. Three days of 0.4 M sucrose pretreatment significantly improved the survival rates of shoot tips immersed in liquid nitrogen. The highest cryopreservation survival rates were obtained with 60-min osmoprotection and 75-min PVS2 dehydration. Survival rates of recalcitrant accessions could be improved with a suitable pretreatment concentration. The sucrose pretreatment concentration was optimized for the intact explants of each accession type (TN66: 0.4 M, TN64: 0.5 M, and TN25: 0.8 M). The highest survival rates of 86.6% (TN66), 76.6% (TN64), and 50.0% (TN25) were achieved using pretreatment for 3 days, loading for 60 min, and dehydration in PVS2 for 75 min. This vitrification procedure with high concentrations of sucrose pretreatment can improve the survival rates of shoot tips after cryopreservation.
cryoprotectionphysiological hardeningplant vitrification solutionornamentalBATATAS L. LAM.GENTIAN AXILLARY BUDSSHOOT-TIPSIPOMOEA-BATATASPRECULTUREREGROWTHSURVIVALPLANTS
NSTL
