首页|A novel microfluidic RNA chip for direct, single-nucleotide specific, rapid and partially-degraded RNA detection
A novel microfluidic RNA chip for direct, single-nucleotide specific, rapid and partially-degraded RNA detection
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
Direct RNA detection is critical for providing the RNA insights into gene expression profiling, noncoding RNAs, RNA-associated diseases and pathogens, without reverse transcription. However, classical RNA analysis usually requires RT-PCR, which can cause bias amplification and quantitation errors. To address this challenge, herein we report a microfluidic RNA chip (the microchip prototype) for direct RNA detection, which is primarily based on RNA extension and labeling with DNA polymerase. This detection strategy is of high specificity (discrimi-nating against single-nucleotide differences), rapidity, accuracy, nuclease resistance, and reusability. Further, we have successfully detected disease-associated RNAs in clinical samples, demonstrating its great potentials in biomedical research and clinical diagnosis.
RNA detectionRNA microfluidic ChipNuclease resistanceCOVID-19 detectionmRNAmiRNA and ncRNA detectionMICRORNA DETECTIONONE-STEPDNACANCER
NSTL
Elsevier
