首页|miR-326 Inhibits Gastric Cancer Cell Growth Through Downregulating NOB1
miR-326 Inhibits Gastric Cancer Cell Growth Through Downregulating NOB1
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NSTL
Cognizant Communication Corp
MicroRNAs (miRNAs) play a crucial role in the development and progression of human cancers， including gastric cancer (GC)。 The discovery of miRNAs may provide a new and powerful tool for studying the mechanism， diagnosis， and treatment of GC。 In this study， we aimed to investigate the role of miR-326 in the development and progression of GC。 Quantitative PCR (qPCR) was used to measure the expression level of miR-326 in GC tissues and cell lines。 We found that miR-326 was significantly downregulated during GC。 In addition， overexpression of miR-326 inhibited GC cell proliferation。 Fluorescence-activated cell sorting (FACS) further showed that miR-326 significantly induced GC cell G2/M arrest。 Subsequent dual-luciferase reporter assay identified one of the proto-oncogene NOB1 as a direct target of miR-326， and NOB1 can save growth inhibition caused by miR-326。 We also confirmed that the growth inhibition caused by miR-326 is associated with AKT pathway activation。 Taken together， our results indicate that miR-326 could serve as a potential diagnostic biomarker and therapeutic option for GC in the near future。
Gastric cancer ( GC)miR-326NOB1ProliferationCell cycleAKT
Ji, Sheqing、Zhang, Bin、Kong, Ye、Ma, Fei、Hua, Yawei
NSTL
Cognizant Communication Corp
