首页|Secretome analysis of Trichoderma reesei RUT-C30 and Penicillium oxalicum reveals their synergic potential to deconstruct sugarcane and energy cane biomasses
Secretome analysis of Trichoderma reesei RUT-C30 and Penicillium oxalicum reveals their synergic potential to deconstruct sugarcane and energy cane biomasses
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NSTL
Elsevier
The conversion of lignocellulosic polymers into monomeric sugars demands a plethora of enzymatic activities generally not produced by a single microorganism and induced by the carbon source. In this vein, this work investigates the synergy between the enzymes secreted by the cellulolytic model fungi Trichoderma reesei RUT C30 (TR) and Penicillium oxalicum (PO) to deconstruct sugarcane straw (SCS) and energy cane bagasse (ECB). TR and PO secrete a similar profile of cellulose-active enzymes resulting in a comparable conversion of SCS and ECB into glucose. The synergy between the enzymes produced by both fungi to break down the cellulose fraction becomes evident by the improvement of glucose titers from similar to 35-54% and from similar to 10-17% in SCS and ECB conditions, respectively, reached with the mixture of the secretomes of both fungi. The effect of a hemicellulaseenriched secretome produced by TR is particularly seen in SCS where the xylose yield reached similar to 15% compared to 5% by PO, remaining unaltered following the mixture of secretomes. However, the secretion of enzymes active in the decorations of the main chain polymers possibly aid PO to access the hemicellulose fraction of ECB reaching xylose yields similar to TR in this condition.
NSTL
Elsevier
