Determination of voriconazole in aqueous humor by liquid chromatography-electrospray ionization-mass spectrometry

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外文摘要：A novel method based on liquid chromatography-mass spectrometry with electrospray ionization (LC-MS) has been developed for analysis of voriconazole in aqueous humor. The separation was achieved on a reversed-phase C_(18) column eluted by 70% acetonitrile-30% water-0.01% TFA. The correlation between the concentration of voriconazole to peak area was linear (r~2 = 0.9990) between 0.04 and 60 ng, with a coefficient of variance of less than 3%. Limit of quantitation (LOQ) was estimated to be 5 ng/ml voriconazole with an injection volume of 2 μl of aqueous humor. Both intra-day and inter-day imprecision were less than 3% over the whole analytical range. Parallel analyses of voriconazole samples by LC-MS and by high-performance liquid chromatography (HPLC)-UV showed that the tow methods were highly correlated (r~2 = 0.9985). LC-MS was used to the determine voriconazole levels achieved in the aqueous humor of the rabbit eye, following topical application of 5 or 10 μg voriconazole in the form of eyedrops for 11 days b.i.d. The lower dosage produced an acqueous humor concentration of 7.29 ± 5.84 μg/ml, while the higher dosage produced a concentration of 14.56 ± 12.90 μg/ml.

外文关键词：

voriconazoleantifungalaqueous humor

作者：

Lei Zhou、Randolph D. Glickman、Nancy Chen、William E. Sponsel、John R. Graybill、Kwok-Wai Lam

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作者单位：

Singapore Eye Research Institute, Singapore National Eye Center, Singapore 168751, Singapore

Department of Ophthalmology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229-0339, USA

Department of Medicine, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229-039, USA

出版年：

2002

Journal of Chromatography, Biomedical Applications

ISSN：0378-4347

年,卷(期)：2002.776(2)