首页|Post-Cardiac Arrest Syndrome Is Not Associated with An Early Bacterial Tanslocation
Post-Cardiac Arrest Syndrome Is Not Associated with An Early Bacterial Tanslocation
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NSTL
Elsevier
Background: The aim of this study was to investigate bacterial translocation and its possible role in the development of post-resuscitation inflammatory response following Cardio-Pulmonary Resuscitation (CPR) after cardiac arrest. Methods: Munich female swine were employed for a model of cardiac arrest via application of electrical current. After 7 min, CPR was initiated, and animals were either successfully return to spontaneous circulation (ROSC) within 40 min or not (no-ROSC). At the end of experimental period and prior to sacrifice, samples from the intestine, mesenteric lymph nodes (MLN), liver and portal vein blood were obtained. Evaluation of inflammation and gut permeability was performed; MLN, liver and portal vein samples were analyzed for 16 s rRNA detection and cytokine mRNA expression. Results: A decreased expression of the tight junction protein Occludin, with higher levels of inflammation, greater epithelial disintegration, ulceration, loss of crypts and villi height were found in the intestines of the ROSC swine in comparison to no-ROSC. The macrophage surface antigen CD-14 staining was relatively more intense in the ROSC than in no-ROSC. Higher levels of TNF-alpha mRNA expression were present in the liver of the ROSC group. Finally, despite the inflammatory response and the gut mucosal alterations in ROSC group, no bacterial translocation was detected in liver, MLN and portal vein. Conclusions: We show that resuscitation from cardiac arrest induces inflammatory response and intestinal permeability in swine 4h after resuscitation, but not a bacterial translocation. Bacterial translocation is not an early phase phenomenon but probably part of the pathophysiologic sequelae. (C) 2021 Elsevier Inc. All rights reserved.
Bacterial translocationIntestinal permeabilityPost-cardiac arrest syndromeOccludinTight junctions16 s rRNAISCHEMIA-REPERFUSION INJURYINFECTIOUS COMPLICATIONSRESUSCITATIONSEVERITYCARDIOPULMONARYENDOTOXEMIABARRIERDEATHCARE
NSTL
Elsevier
