首页|Antimicrobial and immunomodulatory activity of beta-defensin from the Chinese spiny frog (Quasipaa spinosa)
Antimicrobial and immunomodulatory activity of beta-defensin from the Chinese spiny frog (Quasipaa spinosa)
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NSTL
Elsevier
The 8-defensins are important components of the vertebrate innate immune system. While mammalian 8-defensins have wide-ranging antibacterial and immunomodulatory activities, those of amphibians remain largely uncharacterised. In this study, 8-defensin cDNA was identified from the skin transcriptome of the Chinese spiny frog Quasipaa spinosa. This 8-defensin (QS-BD) consists of a signal and a mature peptide. Sequence alignments with other amphibian 8-defensins showed conservation of the functional mature peptide and that its closest relative is 8-defensin from Zhangixalus puerensis. Synthetic QS-BD showed antibacterial activity against Vibrio vulnificus, Vibrio harveyi, Streptococcus iniae, and Aeromonas hydrophila. QS-BD showed bactericidal activity by destroying the cell membrane integrity, but did not hydrolyse genomic DNA. QS-BD treatment promoted respiratory bursts and upregulated the expression of interleukin-18 and tumour necrosis factor-a in the murine leukemic monocyte/macrophage cell line RAW264.7. This is the first demonstration of immunomodulatory activity by an amphibian 8-defensin.
NSTL
Elsevier
