首页|UHPLC–MS/MS method for the determination of azithromycin in human plasma of pediatric patients
UHPLC–MS/MS method for the determination of azithromycin in human plasma of pediatric patients
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NSTL
Abstract The presence of azithromycin in the human plasma of pediatric patients was determined with a UHPLC‐MS/MS assay. Sample preparation was done by protein precipitation, and the separation was achieved on a C18 column by the gradient mixture of the mobile phase A (0.1% acetic acid and 3?mM ammonium acetate in water) and the mobile phase B (0.1% acetic acid and 3?mM ammonium acetate in the solution of acetonitrile, methanol, and water, 47.5/47.5/5, V/V/V). The multiple reaction monitoring mode was adopted to monitor the precursor‐to‐product ion transitions of m/z 749.6?→?m/z 591.5 for azithromycin and m/z 753.6?→?m/z 595.5 for azithromycin‐13C‐d3 (the internal standard) at the positive ionization mode. The calibration curve ranged from 0.5 to 500.0?ng·mL?1, and the correlation coefficient was greater than 0.99. The intra‐ and inter‐batch precision was less than 13.7%. Accuracy determined at four concentrations ranged from 99.5 to 110.8%. The extraction recoveries were more than 95%, and the matrix effects were 98–100%. The stability under various conditions was acceptable with the accuracy deviation within 9.2%. In conclusion, our method was simple, sensitive, and reliable for quantifying azithromycin in plasma among pediatric patients.
NSTL
