Plant Physiology and Biochemistry2022，Vol.1727.DOI:10.1016/j.plaphy.2022.01.013

XAP5 CIRCADIAN TIMEKEEPER specifically modulates 3' splice site recognition and is important for circadian clock regulation partly by alternative splicing of LHY and TIC

Liu, Lei Li, Xiaoyun Yuan, Li Gao, Hui Xu, Xiaodong Zhao, Hongtao Zhang, Guofang
Plant Physiology and Biochemistry2022，Vol.1727.DOI:10.1016/j.plaphy.2022.01.013
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  • NSTL
  • Elsevier

XAP5 CIRCADIAN TIMEKEEPER specifically modulates 3' splice site recognition and is important for circadian clock regulation partly by alternative splicing of LHY and TIC

Liu, Lei 1Li, Xiaoyun 2Yuan, Li 3Gao, Hui 4Xu, Xiaodong 3Zhao, Hongtao 2Zhang, Guofang2
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作者信息

  • 1. Huaiyin Normal Univ
  • 2. Hebei Normal Univ
  • 3. Henan Univ
  • 4. Hebei Normal Univ Sci & Technol
  • 折叠

Abstract

Pre-mRNA splicing is an essential step during gene expression, which takes place in the spliceosome, a large dynamic ribonucleoprotein complex assembled in a stepwise manner. During the last decade, several spliceosomal mutants were functionally identified to cause a lengthened circadian period by introducing intron retention defects into circadian clock genes in Arabidopsis. However, the spliceosomal components that play opposite roles in the circadian period via alternative 3 & PRIME; splice site (Alt 3'ss) are largely unknown. Here, we demonstrated that XCT (XAP5 CIRCADIAN TIMEKEEPER) is a key spliceosomal component associated with multiple splicing factors. Moreover, genome-wide analysis revealed that inactivation of XCT particularly results in defects in Alt 3'ss recognition by RNA sequencing. Further analysis indicated that a strong alteration in the 3 & PRIME; splice sites of LHY and TIC partly accounts for the shortened circadian period of the xct mutant. Therefore, our results demonstrated that mutations in XCT shortened the circadian period partly by alternative splicing of LHY and TIC particularly in 3 & PRIME; splice site recognition, which provides new insight into the link between alternative splicing and the circadian clock.

Key words

Circadian period/Pre-mRNA splicing/Spliceosome/Post-transcriptional regulation/XAP5 CIRCADIAN TIMEKEEPER/TEMPERATURE/RESPONSES/PLANTS/GENES/TIME

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出版年

2022
Plant Physiology and Biochemistry

Plant Physiology and Biochemistry

SCI
ISSN：0981-9428
被引量1
参考文献量28
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