首页|Antioxidant activity-guided isolation of flavonoids from Silene gallica aerial parts
Antioxidant activity-guided isolation of flavonoids from Silene gallica aerial parts
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NSTL
Elsevier
? 2022 Phytochemical Society of EuropeA bio-guided fractionation of the 80% aqueous ethanolic extract of the aerial parts of Silene gallica L. (Caryophyllaceae), growing in North-Eastern Algeria, was performed to evaluate its antioxidant activity using DPPH, hydroxyl radical scavenging and CUPRAC assays. Successive chromatographic separations of the most antioxidant n-BuOH soluble fraction yielded four acylated flavone C-glycosides, vitexin 2''-O-β-D-(4''',6'''-di-acetyl)-glucopyranoside (1), orientin-2''-O-β-D-(4''',6'''-di-acetyl)-glucopyranoside (2), orientin-2''-O-β-D-(6'''-feruloyl)-glucopyranoside (3), and orientin-2''-O-β-D-(6'''-sinapoyl)-glucopyranoside (4), as well as six known compounds including four flavonoids (5-8), a phenylpropanoid glycerolglucoside (regaloside A) (9), and a phytoecdysteroide (20-hydroxyecdysone) (10). Their structures were established by UV, 1D, 2D NMR, and HR-ESI-MS spectral data, in addition to comparison with literature data. The antioxidant activity of the crude extracts, fractions and compounds 1-8 was evaluated. Two acylated orientin glycosides (3 and 4) displayed the strongest antioxidant activity.
AntioxidantCUPRACDPPHFlavonoidHORACSilene gallica L.
Bechkri S.、Khalfallah A.、Kabouche A.、Kabouche Z.、Magid A.A.、Voutquenne-Nazabadioko L.、Sayagh C.、Harakat D.
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Université des frères Mentouri-Constantine 1 département de chimie Laboratoire d′Obtention de Substances Thérapeutiques (LOST)
Université de Reims Champagne Ardenne CNRS ICMR UMR 7312
NSTL
Elsevier
