首页|AuNPs-A beta-Ni-HRP sandwich assay: A new sensitive colorimetric method for the detection of A beta(1-40)
AuNPs-A beta-Ni-HRP sandwich assay: A new sensitive colorimetric method for the detection of A beta(1-40)
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NSTL
Elsevier
Amyloid beta-peptide (A beta) is a key predictor for preclinical diagnosis of Alzheimer's disease (AD) and vascular diseases. In this work, we propose a gold nanoparticle (AuNPs)-A beta-nickel (Ni)-horseradish peroxidase (HRP) based colorimetric assay for the detection of A beta(1-40). The consecutive binding of A beta(1-40) to AuNPs and metal ions is designed and examined for A beta-specific aggregation of AuNPs and the generation of quantitative colorimetric signals. The affinity of A beta(1-40) towards various metal ions was studied first, and two metal ions, Cu and Ni, were specifically tested with Metal Ion-Binding Site Prediction (MIB) and High-resolution Electrospray Ionization Mass Spectrometry (HR-ESI-MS). Subsequently, the binding of A beta(1-40) and AuNPs was examined, and the binding between A beta-AuNPs and Ni-HRP was finally analyzed by UV-Vis and nano-zetasizer. Based on the characterized dual binding of A beta(1-40), a colorimetric sandwich assay was developed and the analytical performance of the developed assay has been evaluated with standard solutions and human serum samples. Good linearity within a range from 0 nM to 10 nM was found. The detection limits of 0.22 nM in the standard sample and 0.23 nM in the human serum sample have been demonstrated. The newly developed colorimetric sandwich assay is a short, simple, antibody-free assay and achieves high sensitivity with only 100 mu L A beta(1-40) samples. The assay has immense potential for the detection of A beta(1-40) in biological or biomedical diagnosis.
NSTL
Elsevier
