Bio-chemical characterization and in silico computational experimental properties of Trianthema triquetra Rottler & Willd.: A desert medicinal plant for industrial products

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NSTL
Elsevier

外文摘要：? 2022 Elsevier B.V.This research work prospects the phytochemical and biological properties of different polarity solvent (methanol, ethyl-acetate, n-hexane, and n-butanol) extracts of Trianthema triquetra Rottler & Willd. The assessment of chemical profile was done by ascertaining total bioactive contents and UHPLC-MS analysis. Similarly, biological profile was analyzed via determination of antioxidant (DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum and metal chelation) and key enzyme inhibition (acetylcholinesterase, butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase) potential. Principal component analysis (PCA) was also conducted to highlight the correlation amongst bioactive contents and tested bioassays. The highest proportion of total phenolic (59.85 mg GAE/g extract) and flavonoid (19.85 mg RE/g extract) contents were exhibited by methanol extract which might be correlated with its higher antioxidant capacity. Similarly, UHPLC-MS phytochemical profiling of the methanol extract unveiled the identification of 26 secondary metabolites belonging to phenolic, flavonoids, glucoside, coumarin, alkaloid, and fatty acid derivatives. The ethyl-acetate fraction exhibited the highest inhibition against BChE, α-amylase and α-glucosidase, while the n-butanol and n-hexane extracts were found to be the active against AChE and tyrosinase enzymes and the molecular docking studies explained the possible mechanism of enzyme inhibition. Results of antioxidant assays were further confirmed by PCA analysis, which shows highly effective association amid total bioactive contents and antioxidant assays.

外文关键词：

AntioxidantEnzyme inhibitionPhytochemicalsTrianthema triquetraUHPLC-MS

作者：

Ahmad S.、Aziz M.、Saleem H.、Wazir M.A.、Sarfraz M.、Anwar S.、Ansari S.A.、Ahmed S.、Mollica A.、Mahomoodally M.F.、Ahemad N.、Khurshid U.

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作者单位：

Department of Pharmacy The Islamia University of Bahawalpur

Institute of Pharmaceutical Sciences (IPS) University of Veterinary & Animal Sciences (UVAS)

Bahawalpur College of Pharmacy Bahawalpur Medical and Dental College

College of Pharmacy Al Ain University

Department of Pharmacology & Toxicology College of Pharmacy University of Hail

Department of Pharmaceutical Chemistry College of Pharmacy King Saud University

Department of Pharmacognosy College of Pharmacy King Saud University

Department of Pharmacy University ‘G. d′Annunzio” of Chieti-Pescara

Department of Health Sciences Faculty of Medicine and Health Sciences University of Mauritius

School of Pharmacy Monash University Malaysia

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出版年：

2022

DOI：

10.1016/j.indcrop.2021.114474

Industrial Crops and Products

EISCI

ISSN：0926-6690

年,卷(期)：2022.177

被引量1
参考文献量49