Tracking of nascent deoxynucleic acids enable by incorporation of uridine variant with 2 prime azidomethyl tag and click chemistry
Zhou, Hongling 1Li, Yuanyuan 1Wang, Sheng 1Wang, Li 1Wang, Rui1
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作者信息
1. Huazhong Univ Sci & Technol
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Abstract
We report here the 2'-azidomethyl modified uridine nucleoside (2'-AmU) can be efficiently synthesized and employed in the labeling study of nascent deoxynucleic acid of interest using fluorophore with alkyne (DBCO-Cy5). This new DNA labeling tool using uridine variant (2'-AmU) displays intensive application potential considering the several advantages it possesses, including readily available, rather small size (minimal perturbation), reversible regulation and easy subjected to click reaction. (C) 2022 Elsevier Ltd. All rights reserved.
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Elsevier