首页|Structural characterization of the family GH115 alpha-glucuronidase from Amphibacillus xylanus yields insight into its coordinated action with alpha-arabinofuranosidases

Structural characterization of the family GH115 alpha-glucuronidase from Amphibacillus xylanus yields insight into its coordinated action with alpha-arabinofuranosidases

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  • NSTL
  • Elsevier
The coordinated action of carbohydrate-active enzymes has mainly been evaluated for the purpose of complete saccharification of plant biomass (lignocellulose) to sugars. By contrast, the coordinated action of accessory hemicellulases on xylan debranching and recovery is less well characterized. Here, the activity of two family GH115 alpha-glucuronidases (SdeAgu115A from Saccharophagus degradans, and AxyAgu115A from Amphibacillus xylanus) on spruce arabinoglucuronoxylan (AGX) was evaluated in combination with an alpha-arabinofuranosidase from families GH51 (AniAbf51A, aka E-AFASE from Aspergillus niger) and GH62 (SthAbf62A from Streptomyces thermoviolaceus). The alpha-arabinofuranosidases boosted (methyl)-glucuronic acid release by SdeAgu115A by approximately 50 % and 30 %, respectively. The impact of the alpha-arabinofuranosidases on AxyAgu115A activity was comparatively low, motivating its structural characterization. The crystal structure of AxyAgu115A revealed increased length and flexibility of the active site loop compared to SdeAgu115A. This structural difference could explain the ability of AxyAgu115A to accommodate more highly substituted arabinoglucuronoxylan, and inform enzyme selections for improved AGX recovery and use.

&945-Arabinofuranosidase&945-GlucuronidaseArabinoglucuronoxylanHemicellulasesGH115GH51GH62

Gatenholm, Paul、Toriz, Guillermo、Tenkanen, Maija、Stogios, Peter J.、Master, Emma R.、Yan, Ruoyu、Wang, Weijun、Vuong, Thu V.、Xiu, Yang、Skarina, Tatiana、Di Leo, Rosa

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Chalmers Univ Technol, Dept Chem & Chem Engn, Wallenberg Wood Sci Ctr, Kemivagen 4, S-41296 Gothenburg, Sweden

Univ Guadalajara, Dept Wood Cellulose & Paper Res, Guadalajara 44100, Jalisco, Mexico

Univ Helsinki, Dept Food & Environm Sci, POB 27, Helsinki 00014, Finland

Univ Toronto, Dept Chem Engn & Appl Chem, 200 Coll St, Toronto, ON M5S 3E5, Canada

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2021

10.1016/j.nbt.2021.01.005

New biotechnology

New biotechnology

EISCI
ISSN：1871-6784
年,卷(期)：2021.62
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