首页|A new phenoxo-bridged dicopper Schiff base complex: Synthesis, crystal structure, DNA/BSA interaction, cytotoxicity assay and catecholase activity
A new phenoxo-bridged dicopper Schiff base complex: Synthesis, crystal structure, DNA/BSA interaction, cytotoxicity assay and catecholase activity
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NSTL
Elsevier
The DNA/BSA interaction and catecholase activity of a new binuclear water-soluble Cu(II) complex [CuII2L(mu 1,1NO3)(mu-OH)(NO3)(H2O)], where L = [2,6-bis{N-(2-pyridylethyl)formidoyl}-4-methylphenol] involving mu-phenoxo N2O4 Schiff base ligand, have been studied. The complex interaction with FS-DNA/BSA was investigated by absorption and fluorescence titration, cyclic voltammetry, circular dichroism, agarose gel electrophoresis and viscosity measurements. The complex is characterized by elemental analysis, UV-vis and FT-IR spectroscopy. Xray analysis revealed the dinuclear Cu(II) ions located in a six-coordinated distorted environment. Docking simulation analysis and competitive binding experiments with ethidium bromide showed the complex interaction with DNA mainly via intercalation mode with the Kb value of 2.78 x 104 M-1. Upon increasing DNA concentration, the fluorescence intensity of the complex enhanced about two folds, and a strong green fluorescence was observed via naked eyes, demonstrating the complex behaves like a "light switch" for DNA in water. Finally, cytotoxic activity was investigated by MTT assay against MCF-7, HeLa and LNCaP cancer cells.
NSTL
Elsevier
