Microbiome-wide association studies between phyllosphere microbiota and ionome highlight the beneficial symbiosis of Lactococcus lactis in alleviating aluminium in cassava

Xu, Haoran ¹Wei, Yunxie ¹Zhang, Jiachao ¹Shi, Haitao ¹Zeng, Hongqiu ¹Hu, Wei ²Liu, Guoyin¹

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作者信息

1. Hainan Univ
2. Chinese Acad Trop Agr Sci
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Abstract

The phyllosphere is one of the most abundant habitats for global microbiota. The ionome is the composition of mineral elements in plants. The correlation between phyllosphere microbiota and the ionome remains elusive in plants, especially in the most important tropical crop cassava. In this study, microbiome-wide association studies (MWASs) of thirty varieties were performed to reveal the association between phyllosphere microbiota and ionomic variations in cassava. Annotation of metagenomic species identified some species that were significantly correlated with ionomic variations in cassava. Among them, Lactococcus lactis abundance was negatively associated with leaf aluminium (Al) levels but positively related to leaf potassium (K) levels. Notably, both the reference and isolated L. lactis showed strong binding capacity to Al. Further bacterial transplantation of isolated L. lactis could significantly decrease endogenous Al levels but increase K levels in cassava, and it can also lead to increased citric acid and lactic acid levels as well as higher transcript levels of K uptake-related genes. Taken together, this study reveals the involvement of phyllosphere microbiota in ionomic variation in cassava, and the correlation between L. lactis abundance and Al and K levels provides novel insights into alleviating Al accumulation and promoting K uptake simultaneously.

Key words

cassava/Phyllosphere microbiota/Ionome/Aluminium (Al)/Potassium (K)/Lactococcus lactis/PLANT/ACCUMULATION/POTASSIUM/GROWTH/ACID/BACTERIA/CADMIUM/SOIL/INOCULATION/TOXICITY

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出版年

2022

Plant Physiology and Biochemistry

SCI

ISSN：0981-9428

参考文献量60

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