首页|MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple
MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
Apple (Malus domestica Borkh。) is not only an important fruit crop distributed worldwide， but also a common model plant。 However， the lack of efficient genetic transformation procedures for apples limits the in-depth studies of their gene functions。 Although leaf-regenerated adventitious shoots (LRAS) are a prerequisite for successful genetic transformation of apple， little is known about the underlying molecular mechanism of LRAS。 Here， we identified the WUSCHEL-related homeobox (WOX) transcription factor in apple， MdWOX4-2， which was a transcriptional activator。 Gene expression as well as morphological and histological observations revealed that MdWOX4-2 is involved in the development of LRAS。 Overexpression of MdWOX4-2 conferred higher regenerative capacity in transgenic tobacco (Nicotiana tabacum) as compared to the wild type (WT)。 The com-bined results of the yeast one-hybrid (Y1H)， electrophoretic mobility shift assay (EMSA)， dual luciferase assays， and transient transactivation assay， revealed that MdWOX4-2 directly bound to and activated the MdLBD41 promoter。 Moreover， transgenic experiments further demonstrated that MdLBD41 could significantly enhance the formation of adventitious shoot in transgenic tobacco。 Collectively， our findings demonstrate that MdWOX4-2 is important for regulating the LRAS development by activating MdLBD41。
NSTL
Elsevier
