Phytochemistry2022，Vol.19410.DOI:10.1016/j.phytochem.2021.113021

Endophytic fungus Cladosporium tenuissimum DF11, an efficient inducer of tanshinone biosynthesis in Salvia miltiorrhiza roots

Chen, Haimin Chen, Jialing Qi, Yao Chu, Siyuan Ma, Yao Xu, Linna Lv, Shiyi Zhang, Haihua Yang, Dongfeng Zhu, Yonghong Mans, Dennis Ra Liang, Zongsuo
Phytochemistry2022，Vol.19410.DOI:10.1016/j.phytochem.2021.113021
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  • NSTL
  • Elsevier

Endophytic fungus Cladosporium tenuissimum DF11, an efficient inducer of tanshinone biosynthesis in Salvia miltiorrhiza roots

Chen, Haimin 1Chen, Jialing 1Qi, Yao 1Chu, Siyuan 1Ma, Yao 1Xu, Linna 1Lv, Shiyi 1Zhang, Haihua 1Yang, Dongfeng 1Zhu, Yonghong 2Mans, Dennis Ra 3Liang, Zongsuo1
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作者信息

  • 1. Zhejiang Sci Tech Univ
  • 2. Tianjin Tasly Holding Grp Co Ltd
  • 3. Anton de Kom Univ Suriname
  • 折叠

Abstract

Salvia miltiorrhiza is a traditional medicinal plant mainly used for cardiovascular and cerebrovascular disease treatment. Tanshinones are the main bioactive constituents of S. miltiorrhiza, which mainly accumulate around its root periderm tissue. Endophytic fungi are important bioelicitors or probiotics that can promote the accumulation of secondary metabolites and sustainable cultivation of medicinal plants. Among them, endophytic Cladosporium spp., possessing a variety of biotransformation and metabolic abilities, is an ideal elicitor source. Here, we used a gnotobiotic system to investigate the effects of the endophytic fungus Cladosporium tenuissimum DF11 on tanshinone biosynthesis in S. miltiorrhiza roots. The results showed that C. tenuissimum DF11 mainly colonizes the intercellular space of the root tissues and promotes tanshinone biosynthesis and accumulation in S. miltiorrhiza roots by upregulating the expression of the genes encoding for key enzymes HMGR, DXS, DXR, GGPPS, CPS, KSL and CYP76AH1 of the tanshinone biosynthesis pathway. The expression levels of almost all genes encoding for key enzymes reached the response peak in the first or second week after DF11 colonization. Taken together, the endophytic fungus C. tenuissimum DF11 could promote secondary metabolite accumulation in S. miltiorrhiza roots. These results indicate that DF11 will be a potential biofertilizer fungus to regulate and stabilize the quality of cultivated S. miltiorrhiza medicinal materials.

Key words

Salvia miltiorrhiza Bunge/Tanshinone/Cladosporium tenuissimum Cooke/Endophytic fungus/Secondary metabolism/HAIRY ROOTS/SYSTEM/IDENTIFICATION/PLATFORMS/ELICITOR/BACTERIA/MIRNAS

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出版年

2022
Phytochemistry

Phytochemistry

CCR
ISSN：0031-9422
被引量6
参考文献量78
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