首页|Utilizing the ?-lactam hydrolyzing activity of ?-lactamase produced by Bacillus cereus EMB20 for remediation of ?-lactam antibiotics
Utilizing the ?-lactam hydrolyzing activity of ?-lactamase produced by Bacillus cereus EMB20 for remediation of ?-lactam antibiotics
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NSTL
Elsevier
? 2021 Elsevier LtdAntibiotic pollution and antibiotic-induced antimicrobial resistance (AMR) are identified as one of the global threats to human and environmental health. Among various antibiotic remediation options, biological methods are considered sustainable and efficient. However, there are limited reports on remediation of high antibiotic loads using biological methods. High antibiotic loads are prevalent in pharmaceutical, aquaculture, and hospital wastewaters. Considering this, herein we explored for the first time the ?-lactamase preparation of Bacillus cereus EMB20 for in-vitro remediation of Ampicillin (Amp) and other ?-lactam antibiotics. Amp concentrations of 50, 100, and 200 mg L?1 were observed to be completely remediated within an hour of treatment, while almost 90% reduction was detected in the case of 300 mg L?1 Amp at the same time period. The enzyme was observed to be active at a broad pH range and showed complete removal of 100 mg L?1 Amp at pH 5, 7, 9. Moreover, the Amp hydrolyzed products were identified using mass spectrometry and the products exhibited no antibiotic and toxic activities. Interestingly, the enzymatic preparation was also active in removing high levels of amoxicillin, meropenem (carbapenem), and the mixture of these two with Amp, within an hour of treatment. In conclusion, the study provides a novel strategy that could be used efficiently for the abatement of ?-lactam-based antibiotic pollution and the consequent spread of AMR in the environment.
NSTL
Elsevier
