Natural product research2022，Vol.36Issue(10/12) ：11.DOI:10.1080/14786419.2021.1899176

Multi-layered antimicrobial synergism of (E)-caryophyllene with minor compounds, tecleanatalensine B and normelicopine, from the leaves of Vepris gossweileri (I. Verd.) Mziray

Moses K.,Langat Yisau,Mayowa Nicholas,Sadgrove Mohammed,Danyaal Thomas A. K.,Prescott Teva,Kami Sianne,Schwikkard James,Barker Martin,Cheek
Natural product research2022，Vol.36Issue(10/12) ：11.DOI:10.1080/14786419.2021.1899176
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Multi-layered antimicrobial synergism of (E)-caryophyllene with minor compounds, tecleanatalensine B and normelicopine, from the leaves of Vepris gossweileri (I. Verd.) Mziray

Moses K.,Langat 1Yisau,Mayowa 1Nicholas,Sadgrove 1Mohammed,Danyaal 1Thomas A. K.,Prescott 1Teva,Kami 2Sianne,Schwikkard 3James,Barker 3Martin,Cheek4
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作者信息

  • 1. Jodrell Laboratory, Natural Capital and Plant Health Department, Royal Botanic Gardens
  • 2. Herbier National, Institut National en Sciences Exactes et Naturelles, (IRSEN), Cité Scientifique
  • 3. School of Life Sciences, Pharmacy and Chemistry, Kingston University
  • 4. Identification and Naming Department, Herbarium, Royal Botanic Gardens
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Abstract

Abstract An aromatic alkaloid-rich ‘absolute’ extract from Vepris gossweileri inhibited Saccharomyces cerevisiae at 62.5?μg.mL?1 and Bacillus subtilis at 500?μg.mL?1 . A loss of activity upon fractionation indicated possible synergistic effects. Three new acridones, gossweicridone A (3), B (4) and C (5) and known compounds from the extract were inactive. Combinations of compounds showed that a sub-fraction containing mixtures of minor compounds with (Ε)-caryophyllene augmented activity by 50-folds, with MIC values of 19.6?μg.mL?1 for S. cerevisiae and 375.0?μg.mL?1 for B. subtilis, demonstrating potent ΣFIC values of 0.02 and 0.375 respectively. From the active sub-fraction, three compounds were assigned as tecleanatalensine B, 13S-hydroxy-9Z,11E,15E-octadecatrienoic acid and normelicopine. In combination with (Ε)-caryophyllene they separately demonstrated MIC values of 18?μg.mL?1, 34?μg.mL?1 and 16?μg.mL?1 , respectively against S. cerevisiae. The synergistic combinations were more potent with addition of pheophytin A, suggesting that the synergistic antifungal effect of the extract is multi-layered.

Key words

Vepris gossweileri (I. Verd.) Mziray/acridone/furoquinoline/Saccharomyces cerevisiae strain BY4743/synergism and potentiation/antimicrobial effects

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出版年

2022
Natural product research

Natural product research

SCI
ISSN：1478-6419
被引量4
参考文献量33
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