首页|Lutein extraction by microemulsion technique: Evaluation of stability versus thermal processing and environmental stresses
Lutein extraction by microemulsion technique: Evaluation of stability versus thermal processing and environmental stresses
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NSTL
Elsevier
The ability of enzyme assisted microemulsion (EAME) technique on lutein extraction from marigold petals powder (MPP) was elucidated. Analyzing the lutein content (LC) of MPP acetone extract confirmed the high accuracy (>98%) of spectrophotometry versus RP-HPLC. Endozym (R) Pectofruit showed better efficiency than Celluclast on lutein extraction with acetone. The efficiency (<15%) of primitive MEs (without co-surfactant) doubled (similar to 28%) on the expense of co-surfactant (1-PrOH). Under the optimized liquification of MPP with pectinase, the extraction efficiency of Span 20 [S20: 1-PrOH (1:5)] ME (LME) dramatically improved (116%), therefore selected for stability evaluation. Results highlighted its superior potential for lutein protection under diverse stresses compared to MPP ethanol extract (MEE). The LC of MEE and LME was gradually decreased over storage time (120 days). Despite the lutein loss (7%) of MEE, LME completely protected its initial loaded lutein under acidified condition. Moreover, during incubation, the LC of MEE (38, 34, 31%) and LME (25, 13 and 9%) was gradually diminished. LC of MEE also completely degraded after 330 min of UV radiation, while LME protected >95% of the initial lutein and only the severe UV radiation (15 cm) led to partially fading (13%).
NSTL
Elsevier
