首页|Nanoencapsulation of hyssop essential oil in chitosan-pea protein isolate nano-complex

Nanoencapsulation of hyssop essential oil in chitosan-pea protein isolate nano-complex

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Nanoencapsulation of bioactive compounds is an emerging field in food and pharmaceutical industries. In present work, essential oil was extracted from aerial parts of hyssop and encapsulated in chitosan-pea protein isolate nano-complex using a nanoprecipitation method. A central composite design (CCD) was employed to optimize the formation of nano-complex for maximizing the retention rate of hyssop essential oil (HEO) loaded in the nano-matrix and minimizing the average particle size simultaneously. The optimum nano-matrix formation were of chitosan/pea protein isolate ratio of 2.12 (w/w) and essential oil/chitosan-pea protein isolate ratio of 1.88 (w/v). Under these conditions, the retention rate of HEO and the average particle size were 81.2% and 216.4 nm, respectively. Furthermore, Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses indicated the success of HEO encapsulation. The formed nanoparticles illustrated a spherical shape and regular distribution as indicated by scanning electron microscopy (SEM). The antioxidant activity of HEO-loaded chitosan-pea protein isolate nano-complex was higher than free HEO. Similarly, the encapsulated HEO had a high antifungal activity against Botrytis cinerea under both in vitro and in vivo conditions. The encapsulated HEO at 2 mg/mL concentration significantly decreased both the incidence and disease severity of gray mold on strawberries during storage at 4 degrees C. These findings imply that HEO-loaded chitosan-pea protein isolate nano-complex is a promising case to be used for novel applications in pharmaceutical and food industries.

Bioactive compoundsPea protein isolatePreservationEncapsulationStructural characterizations

Hadidi, Milad、Motamedzadegan, Ali、Jelyani, Aniseh Zarei、Khashadeh, Sanaz

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Univ Lleida, Dept Food Technol, Av Rovira Roure 191, Lleida 25198, Spain

Sari Agr Sci & Nat Resources Univ, Dept Food Sci & Technol, Sari, Mazandaran, Iran

Shiraz Univ Med Sci, Dept Food & Drug, Food Control Lab, Shiraz, Iran

Tajan High Educ Inst, Dept Food Sci, Ghaemshahr, Mazandaran, Iran

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2021

LWT-Food Science & Technology

LWT-Food Science & Technology

ISSN:0023-6438
年,卷(期):2021.144
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