Garlic extract was evaluated in the mouse bone marrow micronucleus test for its possible protective effects against gamma-radiation-induced chromosomal damage. Together with this, biochemical assays were carried out to determine the changes in sulfhydryl content and glutathione S-transferase activities. Three doses of freshly prepared garlic extract (125, 250 and 500 mg/kg b.w.) were orally administered for 5 consecutive days, and the animals were irradiated 2 h after the final feeding. The results of the micronucleus test demonstrated that pre-treatment with garlic extract can lead to significant dose-related reductions in the frequencies of gamma-radiation-induced (2 Gy) micronucleated polychromatic erythrocytes. The anticlastogenic effect of garlic extract was observed against lower radiation doses of 0.5 and 1 Gy, but not 0.25 Gy. Significant increases in the sulfhydryl content and glutathione S-transferase activity were observed after either pre-treatment with garlic extract or irradiation. However, the irradiated garlic-extract pre-treated animals showed a significant reduction in sulfhydryl content and glutathione S-transferase activities.
Garlic extractGlutathione s transferaseMicronucleus testBindingCancerAcidDna
Singh SP、Abraham SK、Kesavan PC
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Abraham SK, JAWAHARLAL NEHRU UNIV, SCH LIFE SCI, NEW DELHI 110067, INDIA
NSTL
