首页|Poly‐L‐lysine/hyaluronan nanocarriers as a novel nanosystem for gene delivery

Poly‐L‐lysine/hyaluronan nanocarriers as a novel nanosystem for gene delivery

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  • NSTL
  • Wiley
Abstract The present research comes up with a novel DNA‐loaded poly‐L‐lysine (PLL)/hyaluronan (HA) nanocarrier (DNA‐loaded PLL/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA‐loaded PLL molecules by HA. Fourier‐transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission‐scanning electron microscopy?(FE‐SEM) and transmission electron microscopy (TEM) were used to analyse the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of –24 ± 3?mV, with an average size of 138 ± 6 nm, in an ellipsoid‐shape with smooth surfaces. The DNA loading efficiency (LE) measured by DNA absorbance was around 95 %. The MTT assay showed that the developed NCs are non‐toxic to the cells. Furthermore, the uptake of the DNA‐loaded PLL/HA NCs by the human embryonic kidney (HEK)‐293T cells was evaluated by a flow cytometry method, and demonstrated high potential cellular uptake over 90% for transferring the gene to HEK‐293T cells at the optimised conditions. Therefore, the DNA‐loaded PLL/HA NCs are the potent strategy for developing nanosystems for gene delivery applications.

gene deliveryhyaluronannanocarrierspoly‐L‐lysine

Masoumeh Souri、Mohammad Aref Bagherzadeh、Mirza Ali Mofazzal Jahromi、Hossein Mohammad‐Beigi、Amir Abdoli、Hamed Mir、Abazar Roustazadeh、Majid Pirestani、Parham Sahandi Zangabad、Jafar Kiani、Amirmahmoud Bakhshayesh、Mehdi Jahani、Mohammad Taghi Joghataei、Mahdi Karimi

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Cellular and Molecular Research Center,Iran University of Medical Sciences

Student Research Committee,Jahrom University of Medical Sciences

Research Center for Noncommunicable Diseases,Jahrom University of Medical Sciences

Department of Biotechnology and Biomedicine,Technical University of Denmark

Department of Parasitology, and Mycology, School of Medicine,Jahrom University of Medical Sciences

Department of Biochemistry and Nutrition, School of Medicine,Jahrom University of Medical Sciences

Faculty of Medical Sciences,Tarbiat Modares University

Advances Nanobiotechnology and Nanomedicine Research Group (ANNRG),Iran University of Medical

Faculty of Advanced Technologies in Medicine,Iran University of Medical Sciences

Department of Physics,Sharif University of Technology

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2022

10.1111/jmi.13107

Journal of Microscopy

Journal of Microscopy

EISCI
ISSN：0022-2720
年,卷(期)：2022.287(1)
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