首页|Efficient production of isoquercitin, icariin and icariside II by a novel thermostable a-l-rhamnosidase PodoRha from Paenibacillus odorifer with high a-1, 6-/a-1, 2-glycoside specificity

Efficient production of isoquercitin, icariin and icariside II by a novel thermostable a-l-rhamnosidase PodoRha from Paenibacillus odorifer with high a-1, 6-/a-1, 2-glycoside specificity

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  • NSTL
  • Elsevier
Minor flavonoids, such as isoquercitin, icariin and icariside II, have desired bioactivity and bioavailability. To enhance the production efficiency on converting rutin and epimedin C into minor flavonoids, a novel GH family 78 alpha-L-rhamnosidase PodoRha from the Paenibacillus odorifer was cloned and heterologous over-expressed in E. coli BL21(DE3). PodoRha exhibited a high catalytic ability in cleaving outer alpha-1, 2-rhamnopyranosyl moieties of epimedin C and alpha-1, 6-rhamnopyranosyl of rutin. It displayed the highest activity at 45 ? and pH 6.5, and has great thermostability at 40 ?. Under the optimal transformation condition (40 ?, pH 6.5, 1 h incubation), 10 g/L of Rutin was 99.86% transformed into 6.95 g/L of isoquercitin by PodoRha with a crude productivity of 6.95 g.L-1.h(-1); 1.964 g/L icariin was detected after the epimedin C of the total Epimedium flavonoids (10 g/L) were 99.98% converted by PodoRha. Moreover, by using a two-stage conversion system with a crude icariside II productivity of 2.081 g.L-1.h(-1), 5 g/L epimedin C was 99.85% converted into 3.122 g/L of icariside II with 1 h incubation under pH 6.5 at 40 ? after addition of 5.5 U/mL PodoRha; then with 30 min incubation under pH 6.5 at 90 ? after addition of 0.8 U/mL IagBgl1.

Flavonoid bioconversiona-l-rhamnosidaseThermostabilityIsoquercitinIcariinIcariside IIALPHA-L-RHAMNOSIDASEBIOCHEMICAL-CHARACTERIZATIONCRYSTAL-STRUCTUREASPERGILLUS-TERREUSPURIFICATIONBIOTRANSFORMATIONIMMOBILIZATIONGLYCOSIDASESNARINGINASEEPIMEDIUM

Xie, Jingcong、Zhao, Jian、Zhang, Ning、Xu, Hao、Yang, Jing、Ye, Jun、Jiang, Jianchun

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Chinese Acad Forestry

2022

10.1016/j.enzmictec.2022.110039

Enzyme and Microbial Technology

Enzyme and Microbial Technology

EISCI
ISSN：0141-0229
年,卷(期)：2022.158
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