首页|Cassava Starch: Exploring its Potential as an Alternative Gelling Agent for In; vitro Regeneration and Multiplication of Sweet Potato Plantlets
Cassava Starch: Exploring its Potential as an Alternative Gelling Agent for In; vitro Regeneration and Multiplication of Sweet Potato Plantlets
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NSTL
Murashige and Skoog basal media gelled with agar, gelrite and different cassava starches, supplemented with 30g/l sucrose were investigated for regeneration and in vitro propagation of sweet potato plantlets. Highest shoot height, leaves and node increases of 10.7±2.5 cm, 15.5±1.4 and 14.5=1=1.6 were obtained from TMS 98/0505, TMS 92/0057 and TMS 92/00057 newly processed starch-gelled media compared to 13.6±2.7 cm, 19±2.1 and 17.7±2.0 from gelrite-gelled media after 8 weeks of culture. After 3 years of storage, TMS 30572 and TMS 98/0505 starch-gelled media improved dramatically in enhancing regeneration and propagation. Consequently, TMS 30572 starch-gelled medium improved from 4.2±0.04 to 13.1 ±2.4 cm shoot height, 6.6±0.6 tol3.8±4.8 and 6.1±0.5 to 13.0=1=3.5 for leaves and nodes respectively. Similar increases were also obtained from TMS 98/0505 starch-gelled medium with shoot height increase of 10.7=1=1.5 to 15.0±2.2 cm, 13.8±5.5 to 15.7±6.6 and 12.9=1=1.8 to 14.7=1=5.7 for leaf andnode growth respectively. Although TMS 98/0505 starch-gelled medium performed better than the controls with 15.0±2.2 cm in shoot height increase after storage, gelrite and agar-gelled media proved to be the most efficient for in vitro regeneration andmultiplication.
Alfred O. Ubalua、Carol I. Ihezie、Ahamefula I. Ikpeama
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Plant Tissue Culture Unit, Biotechnology Research and Development Center, National Root Crops Research Institute (NRCRI) Umudike, PMB 7006 Umuahia, Abia State, Nigeria
NSTL
