首页|Hemicellulose-derived sugars solubilisation of rape straw. Cofermentation of pentoses and hexoses by Escherichia coli
Hemicellulose-derived sugars solubilisation of rape straw. Cofermentation of pentoses and hexoses by Escherichia coli
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NSTL
Spanish Natl Inst Agricultural & Food Research & Technolo
Bioconversion of hemicellulose sugars is essential for increasing fuel ethanol yields from lignocellulosic biomass. We report for the first time with rape straw, bioethanol production from hemicellulose sugars. Rape straw was pretreated at mild conditions with sulfuric acid to solubilize the hemicellulose fraction. This pretreatment allows obtaining a prehydrolysate, consisting basically in a solution of monomeric hemicellulosic sugars, with low inhibitor concentrations. The remaining water insoluble solid constitutes a cellulose-enriched, free of extractives material. The influence of temperature (120 degrees C and 130 degrees C), acid concentration (2-4% w/v) and pretreatment time (30-180 min) on hemicellulose-derived sugars solubilisation was evaluated. The highest hemicellulosic sugars recovery, 72.3%, was achieved at 130 degrees C with 2% sulfuric acid and 60 min. At these conditions, a concentrated sugars solution, 52.4 g/L, was obtained after three acid consecutive contacts, with 67% xylose and acetic acid concentration above 4.5 g/L. After a detoxification step by activated charcoal or ion-exchange resin, prehydrolysate was fermented by ethanologenic Escherichia coli. An alcoholic solution of 25 g/L and 86% of theoretical ethanol yield was attained after 144 h when the prehydrolysate was detoxified by ion-exchange resin. The results obtained in the present work show sulfuric acid pretreatment under mild conditions and E. coli as an interesting process to exploit hemicellulosic sugars in rape straw.
agricultural residueacid pretreatmentxyloseE. colibioethanol production
Carlos Lopez-Linares, Juan、Cara-Corpas, Cristobal、Ruiz-Ramos, Encarnacion、Moya-Vilar, Manuel、Castro-Galiano, Eulogio、Romero-Pulido, Inmaculada
NSTL
Spanish Natl Inst Agricultural & Food Research & Technolo
