首页|Identification of Hemoglobin Variants Prevalent in China and Their Effects on Hemoglobin A(1c) Measurements
Identification of Hemoglobin Variants Prevalent in China and Their Effects on Hemoglobin A(1c) Measurements
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NSTL
Oxford Univ Press
Objectives We aimed to evaluate the effects of hemoglobin (Hb) variants prevalent in China on HbA(1c) measurements and to identify them during HbA(1c) measurements. Methods We evaluated a cation-exchange high-performance liquid chromatography (HPLC) method (Bio-Rad D-100), a capillary electrophoresis (CE) method (Capillarys 3 TERA), an immunoassay (Cobas c501), and a boronate affinity method (Premier Hb9210, as a comparative method) for HbA(1c) measurements in the presence of Hb variants prevalent in China. Results The Bio-Rad D-100 and Capillarys 3 TERA gave specific retention times and numeric migration positions for each Hb variant, respectively, showing excellent interindividual reproducibility. All methods showed statistically significant differences (P < .01) for several variants. Clinically significant effects were observed for the Bio-Rad D-100 (Hb New York and Hb J-Bangkok), Capillarys 3 TERA (Hb New York and Hb J-Bangkok), and Cobas c501 (Hb New York). Among 297 samples with Hb variants, there were 75 (25.3%) unacceptable results for Bio-Rad D-100, 28 (9.4%) for Capillarys 3 TERA, and 19 (6.4%) for Cobas c501 compared with the results from Premier Hb9210. Conclusions Some Hb variants prevalent in China affect HbA(1c) measurements. The HPLC retention time and CE migration position can aid in the presumptive identification of Hb variants.
NSTL
Oxford Univ Press
