首页|COMPARISON OF THE SENSITIVITIES OF CRUSTACEAN SPECIES TO RECOMBINANT PHOTORHABDUS INSECT-RELATED (PIR) TOXINS OF ACUTE HEPATOPANCREATIC NECROSIS DISEASE-CAUSING VIBRIO PARAHAEMOLYTICUS
COMPARISON OF THE SENSITIVITIES OF CRUSTACEAN SPECIES TO RECOMBINANT PHOTORHABDUS INSECT-RELATED (PIR) TOXINS OF ACUTE HEPATOPANCREATIC NECROSIS DISEASE-CAUSING VIBRIO PARAHAEMOLYTICUS
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Brill
Photorhabdus insect-related PirAvp (where vp stands for Vibrio parahaemolyticus) and PirBvp of Vibrio parahaemolyticus were expressed in Escherichia coli, designated rPirAvp and rPirBvp, respectively, then tested on 4 crustaceans via immersion tests. PirBvp toxin was detected from 2 h after incubation and accumulated with bacterial growth. The culture filtrate of 15 nM PirBvp toxin caused 100% mortality of Litopenaeus vannamei within 4 days after immersion. Litopenaeus vannamei showed 100% mortality at 7 days after immersion in purified 4 nM rPirAvp, rPirBvp, or rPirAvp + rPirBvp mixture. The cumulative mortalities in the rPirAvp, rPirBvp or rPirAvp + rPirBvp mixture were 50, 83 and 100% in Macrobrachium rosenbergii after 12 days of immersion, 11, 77 and 100% in Palaemon paucidens after 10 days of immersion, and 36, 96 and 100% in Macrophthalmus japonicus after 6 days of immersion, respectively.
AHPNDcrustaceanssensitivityrecombinant Pir toxinsAGENTPROTEINSTRAIN
Lee, Miyoung、Kim, Suhyun、Jang, In-Kwon、Kim, Ji Seok、Uhm, Taewoong、Yi, Seongbaek、Choi, Tae-Jin
NSTL
Brill
