首页|Detection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike protein

Detection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike protein

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  • NSTL
  • Elsevier
Mass testing for the diagnosis of COVID-19 has been hampered in many countries owing to the high cost of genetic material detection. This study reports on a low-cost immunoassay for detecting SARS-CoV-2 within 30 min using dynamic light scattering (DLS). The immunosensor comprises 50-nm gold nanoparticles (AuNPs) functionalized with antibodies against SARS-CoV-2 spike glycoprotein, whose bioconjugation was confirmed using transmission electron microscopy (TEM), UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and surface-enhanced Raman scattering spectroscopy (SERS). The specific binding of the bioconjugates to the spike protein led to an increase in bioconjugate size, with a limit of detection (LOD) 5.29 x 10(3) TCID50/mL (Tissue Culture Infectious Dose). The immunosensor was also proven to be selective upon interaction with influenza viruses once no increase in size was observed after DLS measurement. The strategy proposed here aimed to use antibodies conjugated to AuNPs as a generic platform that can be extended to other detection principles, enabling technologies for low-cost mass testing for COVID-19.

SARS-CoV-2ImmunosensorsBioconjugatesSpectroscopic techniquesInfluenza virusSURFACE

da Silva, Patricia Bento、da Silva, Jaqueline Rodrigues、Rodrigues, Mosar Correa、Vieira, Julia Augusto、de Andrade, Ikaro Alves、Nagata, Tatsuya、Santos, Alexandre Silva、da Silva, Sebastiao William、Oliveira da Rocha, Marcia Cristina、Bao, Sonia Nair、Moraes-Vieira, Pedro M.、Proenca-Modena, Jose、Angelim, Monara K. C.、de Souza, Gabriela Fabiano、Muraro, Stefanie Primon、Branco de Barros, Andre Luis、Ribeiro-Dias, Fatima、Machado, Giovanna、Fessel, Melissa Regina、Chudzinski-Tavassi, Ana Marisa、Ronconi, Celia Machado、Goncalves, Debora、Curi, Rui、Oliveira Jr, Osvaldo N.、Azevedo, Ricardo Bentes、de Souza Martins, Glendara Aparecida

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Univ Brasilia

Univ Estadual Campinas

Univ Fed Minas Gerais

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Ctr Tecnol Estrateg Nordeste

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Fed Univ Tocantins

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2022

10.1016/j.talanta.2022.123355

Talanta

Talanta

EISCI
ISSN：0039-9140
年,卷(期)：2022.243
  • 9
  • 43