首页|Soybean hull valorization for sugar production through the optimization of citric acid pretreatment and enzymatic hydrolysis
Soybean hull valorization for sugar production through the optimization of citric acid pretreatment and enzymatic hydrolysis
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NSTL
Elsevier
? 2022 Elsevier B.V.Soybean hull is a worldwide produced lignocellulosic waste from the soybean industrial sector. Its exploration through biotechnological approaches could increment considerably the value and economic return of soybean production-chain. The present study evaluated a citric acid pretreatment, an organic acid with eco-friendly characteristics and low inhibitors generation, in the valorization of soybean hull for fermentable sugars recovery. The optimized condition through response surface methodology was 4.2 % (w:w) citric acid, LSR of 5, 105 min, and 120 oC, with 80.42 % of mass yield and a very high recovery of cellulose fraction. Subsequent enzymatic hydrolysis was evaluated varying solid contents (10–20 %) and enzyme loading (10–20 FPU/g), reaching 72.28–97.10 % enzymatic conversions and 36.52–72.03 g/L glucose final titers. Final proposed process resulted in high enzymatic conversion of 82.86 %, with glucose and xylose final titers of 72.03 and 31.13 g/L, respectively. Citric acid pretreatment has shown to be effective to improve soybean hull conversion into sugars, resulting in a process that can be potential applied in further fermentation processes for the production of biomolecules in a biorefinery context.
NSTL
Elsevier
