Enhanced production of gamma-aminobutyric acid (GABA) using Lactobacillus plantarum EJ2014 with simple medium composition

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NSTL
Elsevier

外文摘要：gamma-Aminobutyric acid (GABA) is an amino acid not present in proteins. GABA participates in a variety of physiological functions and has received significant attention in the medical and food industries. In this study, GABA production was investigated using Lactobacillus plantarum. We designed a synthetic medium consisting of the simplest medium components such as yeast extract (nitrogen source), glucose (carbon source), and monosodium L-glutamate (MSG; substrate for GABA) and optimized their proportions to efficiently produce GABA. As a result, 19.8 g/L GABA was obtained under an optimal synthetic medium composition of 100 g/L yeast extract, 10 g/L glucose, and 2.25% MSG, which is the highest among previous studies using L. plantarum. Interestingly, 159.7% of theoretical maximum yield based on initial MSG content was obtained since yeast extract acts as a secondary source of substrate. By proper optimization with the simplest medium using L. plantarum, it can produce a high titer of GABA as well as might help various functional foods containing GABA to develop.

外文关键词：

gamma-Aminobutyric acidLactobacillus plantarumGlutamate decarboxylaseMonosodium L-glutamateYeast extractMedia optimization

作者：

Park, Su Jeong、Kim, Dong Hyun、Kang, Hye Jee、Shin, Minhye、Yang, Soo-Yeon、Yang, Jungwoo、Jung, Young Hoon

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作者单位：

Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu 41566, South Korea

Korea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South Korea

Seoul Natl Univ, Res Inst Agr & Life Sci, Dept Agr Biotechnol, Seoul 08826, South Korea

Ildong Biosci, 17 Poseunggongdan Ro, Pyeongtaek Si 17957, Gyeonggi Do, South Korea

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出版年：

2021

DOI：

10.1016/j.lwt.2020.110443

LWT-Food Science & Technology

ISSN：0023-6438

年,卷(期)：2021.137

被引量19
参考文献量40