Abstract
Euchresta tubulosa Dunn.is a Chinese herbal medicine with biological activity,but there are few studies on its components at present.Alkaloids in the stem of Euchresta tubulosa Dunn.were isolated and purified by high-speed counter-current chromatography(HSCCC)using stepwise elution.First of all,liquid-liquid extraction(methylene chloride-methanol-water,5:1:4,v/v)was used for the preliminary enrichment.According to the partition coefficient(K)of a target compound in a series of different two-phase solvents,the final result was that carbon tetrachloride-methylene chloride-methanol-water(2:3:3:2,v/v)(1)and methylene chloride-methanol-water(5:3:2,v/v)(2)were suitable for the HSCCC using stepwise elution.As a result,the purity was all higher than 93% and matrine(1),oxymatrine(2),N-formyl cytisine(3),and N-acetyl cytisine(4)can be eluted at one time by this mode.Cytisine-type alkaloids were isolated for the first time in this plant.Finally,the applicability of the mode was verified.
NSTL
Mdpi