首页|Effects of pretreatments on physicochemical and structural properties of proteins isolated from canola seeds after oil extraction by supercritical-CO2 process
Effects of pretreatments on physicochemical and structural properties of proteins isolated from canola seeds after oil extraction by supercritical-CO2 process
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NSTL
Elsevier
The present research was to investigate the influences of pretreatments on the structural and physicochemical properties of proteins isolated from canola seeds (CSP) after oil extraction by supercritical CO2 fluid. The results showed that the CSPs pretreated with microwave treatment (MV-CSP) and ultrasound treatment (UL-CSP) significantly differed in terms of molecular weight distribution, amino acid composition, and the primary conformation of CSP. The crystalline properties and secondary structural conformation as well as average particle sizes of CSPs were not significantly influenced by pretreatments, but the morphological characteristics were remarkably changed. The physicochemical properties including solubility, water/oil holding capacity, emulsifying capacity and stability, as well as foaming capacity and stability were substantially altered by the pretreatments. The MV-CSP and UL-CSP possessed better physicochemical characteristics. Therefore, microwaveand ultrasound-pretreatments could serve as potential technologies to improve the physicochemical properties of canola seeds proteins. Moreover, different oil extraction methods greatly influenced the quality and functional properties of isolated CSPs. The supercritical CO2 (SC-CO2) process protected intact functional properties and enhanced the overall quality of the isolated CSPs compared to solvent extraction.
PretreatmentPhysicochemical propertyStructural propertyCanola seeds protein
Li, Xiaoyu、Shi, John、Xue, Sophia Jun、Lu, John、Scanlon, Martin
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Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada
Agr & Agri Food Canada, Lethbridge Res & Dev Ctr, Lethbridge, AB, Canada
NSTL
Elsevier
